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High-Throughput Biophysical Analysis and Data Visualization of Conformational Stability of an IgG1 Monoclonal Antibody (mAb) After Deglycosylation

The structural integrity and conformational stability of an IgG1 monoclonal antibody (mAb), after partial and complete enzymatic removal of the N-linked Fc glycan, was compared to the untreated mAb over a wide range of temperature (10° to 90°C) and solution pH (3 to 8) using circular dichroism, fluo...

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書誌詳細
主要な著者: Alsenaidy, Mohammad A., Kim, Jae Hyun, Majumdar, Ranajoy, Weis, David D., Joshi, Sangeeta B., Tolbert, Thomas J., Middaugh, C. Russell, Volkin, David B.
フォーマット: Artigo
言語:Inglês
出版事項: 2013
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC3832129/
https://ncbi.nlm.nih.gov/pubmed/24114789
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/jps.23730
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