Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation

Components of the prokaryotic clustered regularly interspersed palindromic repeat (CRISPR) loci have recently been repurposed for use in mammalian cells(1–6). The Cas9 protein can be programmed with a single guide RNA (sgRNA) to generate site-specific DNA breaks, but there are few known rules govern...

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Vydáno v:Nat Biotechnol
Hlavní autoři: Doench, John G., Hartenian, Ella, Graham, Daniel B., Tothova, Zuzana, Hegde, Mudra, Smith, Ian, Sullender, Meagan, Ebert, Benjamin L., Xavier, Ramnik J., Root, David E.
Médium: Artigo
Jazyk:Inglês
Vydáno: 2014
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4262738/
https://ncbi.nlm.nih.gov/pubmed/25184501
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nbt.3026
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