Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9

CRISPR-Cas9-based genetic screens are a powerful new tool in biology. By simply altering the sequence of the single-guide RNA (sgRNA), Cas9 can be reprogrammed to target different sites in the genome with relative ease, but the on-target activity and off-target effects of individual sgRNAs can vary...

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Publié dans:Nat Biotechnol
Auteurs principaux: Doench, John G., Fusi, Nicolo, Sullender, Meagan, Hegde, Mudra, Vaimberg, Emma W., Donovan, Katherine F., Smith, Ian, Tothova, Zuzana, Wilen, Craig, Orchard, Robert, Virgin, Herbert W., Listgarten, Jennifer, Root, David E.
Format: Artigo
Langue:Inglês
Publié: 2016
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Article
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4744125/
https://ncbi.nlm.nih.gov/pubmed/26780180
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nbt.3437
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