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Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9
CRISPR-Cas9-based genetic screens are a powerful new tool in biology. By simply altering the sequence of the single-guide RNA (sgRNA), Cas9 can be reprogrammed to target different sites in the genome with relative ease, but the on-target activity and off-target effects of individual sgRNAs can vary...
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| Pubblicato in: | Nat Biotechnol |
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| Autori principali: | , , , , , , , , , , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
2016
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4744125/ https://ncbi.nlm.nih.gov/pubmed/26780180 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nbt.3437 |
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