Optimized libraries for CRISPR-Cas9 genetic screens with multiple modalities

The creation of genome-wide libraries for CRISPR knockout (CRISPRko), interference (CRISPRi), and activation (CRISPRa) has enabled the systematic interrogation of gene function. Here, we show that our recently-described CRISPRko library (Brunello) is more effective than previously published librarie...

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Vydáno v:Nat Commun
Hlavní autoři: Sanson, Kendall R., Hanna, Ruth E., Hegde, Mudra, Donovan, Katherine F., Strand, Christine, Sullender, Meagan E., Vaimberg, Emma W., Goodale, Amy, Root, David E., Piccioni, Federica, Doench, John G.
Médium: Artigo
Jazyk:Inglês
Vydáno: Nature Publishing Group UK 2018
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6303322/
https://ncbi.nlm.nih.gov/pubmed/30575746
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-018-07901-8
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