Creation of Novel Protein Variants with CRISPR/Cas9-Mediated Mutagenesis: Turning a Screening By-Product into a Discovery Tool

CRISPR/Cas9 screening has proven to be a versatile tool for genomics research. Based on unexpected results from a genome-wide screen, we developed a CRISPR/Cas9-mediated approach to mutagenesis, exploiting the allelic diversity generated by error-prone non-homologous end-joining (NHEJ) to identify n...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Donovan, Katherine F., Hegde, Mudra, Sullender, Meagan, Vaimberg, Emma W., Johannessen, Cory M., Root, David E., Doench, John G.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2017
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5261743/
https://ncbi.nlm.nih.gov/pubmed/28118392
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0170445
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