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Uncoupling of sgRNAs from their associated barcodes during PCR amplification of combinatorial CRISPR screens
Many implementations of pooled screens in mammalian cells rely on linking an element of interest to a barcode, with the latter subsequently quantitated by next generation sequencing. However, substantial uncoupling between these paired elements during lentiviral production has been reported, especia...
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| Publicado no: | PLoS One |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5969736/ https://ncbi.nlm.nih.gov/pubmed/29799876 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0197547 |
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