Function-based gene identification using enzymatically generated normalized shRNA library and massive parallel sequencing

As a general strategy for function-based gene identification, an shRNA library containing ≈150 shRNAs per gene was enzymatically generated from normalized (reduced-redundance) human cDNA. The library was constructed in an inducible lentiviral vector, enabling propagation of growth-inhibiting shRNAs...

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Bibliografiske detaljer
Main Authors: Shtutman, Michael, Maliyekkel, Anil, Shao, Yu, Carmack, C. Steven, Baig, Mirza, Warholic, Natalie, Cole, Kelly, Broude, Eugenia V., Harkins, Timothy T., Ding, Ye, Roninson, Igor B.
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 2010
Fag:
Biological Sciences
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2867740/
https://ncbi.nlm.nih.gov/pubmed/20368428
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1003055107
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