Rapid Creation and Quantitative Monitoring of High Coverage shRNA Libraries

Short hairpin RNA (shRNA) libraries are limited by the low efficacy of many shRNAs, giving false negatives, and off-target effects, giving false positives. Here we present a strategy for rapidly creating expanded shRNA pools (∼30 shRNAs/gene) that are analyzed by deep-sequencing (EXPAND). This appro...

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Autori principali: Bassik, Michael C., Lebbink, Robert Jan, Churchman, L. Stirling, Ingolia, Nicholas T., Patena, Weronika, LeProust, Emily M., Schuldiner, Maya, Weissman, Jonathan S., McManus, Michael T.
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2009
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Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2783737/
https://ncbi.nlm.nih.gov/pubmed/19448642
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1330
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