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PCR-based generation of shRNA libraries from cDNAs
BACKGROUND: The use of small interfering RNAs (siRNAs) to silence target gene expression has greatly facilitated mammalian genetic analysis by generating loss-of-function mutants. In recent years, high-throughput, genome-wide screening of siRNA libraries has emerged as a viable approach. Two differe...
Tallennettuna:
| Päätekijät: | , , , , , |
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| Aineistotyyppi: | Artigo |
| Kieli: | Inglês |
| Julkaistu: |
BioMed Central
2006
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| Aiheet: | |
| Linkit: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1533825/ https://ncbi.nlm.nih.gov/pubmed/16790063 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-6-28 |
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