PCR-based generation of shRNA libraries from cDNAs

BACKGROUND: The use of small interfering RNAs (siRNAs) to silence target gene expression has greatly facilitated mammalian genetic analysis by generating loss-of-function mutants. In recent years, high-throughput, genome-wide screening of siRNA libraries has emerged as a viable approach. Two differe...

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Detaylı Bibliyografya
Asıl Yazarlar: Du, Cheng, Ge, Baosheng, Liu, Zhongfeng, Fu, Kai, Chan, Wing C, McKeithan, Timothy W
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: BioMed Central 2006
Konular:
Methodology Article
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC1533825/
https://ncbi.nlm.nih.gov/pubmed/16790063
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-6-28
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