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PCR-based immortalization and screening of hierarchical pools of cDNAs.
Starting from sequences of at least 60 bp, PCR-based screening has been developed to recover cDNAs from libraries without the necessity for hybridization or extensive DNA extraction steps. The method maintains the indefinite availability of even scarce cDNA libraries and provides an estimate of the...
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| 出版年: | Nucleic Acids Res |
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| 主要な著者: | , , , , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Oxford University Press
1994
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| オンライン･アクセス: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC308534/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7984433/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/22.22.4806 |
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