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PCR-based immortalization and screening of hierarchical pools of cDNAs.

Starting from sequences of at least 60 bp, PCR-based screening has been developed to recover cDNAs from libraries without the necessity for hybridization or extensive DNA extraction steps. The method maintains the indefinite availability of even scarce cDNA libraries and provides an estimate of the...

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Bibliografske podrobnosti
izdano v:Nucleic Acids Res
Main Authors: D'Esposito, M, Mazzarella, R, Pengue, G, Jones, C, D'Urso, M, Schlessinger, D
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 1994
Online dostop:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC308534/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7984433/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/22.22.4806
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