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A rapid and simple PCR-based method for isolation of cDNAs from differentially expressed genes.
Recently two techniques have been reported which use arbitrarily primed RT-PCR amplification of cDNA fragments from subsets of mRNAs to detect cDNA fragments from differentially expressed mRNAs. Here we report a simple and rapid PCR-based protocol to both detect and isolate cDNA fragments of up to 3...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
1994
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| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC308403/ https://ncbi.nlm.nih.gov/pubmed/7524031 |
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