A rapid and simple PCR-based method for isolation of cDNAs from differentially expressed genes.
Recently two techniques have been reported which use arbitrarily primed RT-PCR amplification of cDNA fragments from subsets of mRNAs to detect cDNA fragments from differentially expressed mRNAs. Here we report a simple and rapid PCR-based protocol to both detect and isolate cDNA fragments of up to 3...
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| Veröffentlicht in: | Nucleic Acids Res |
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| Hauptverfasser: | , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Oxford University Press
1994
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| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC308403/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7524031/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/22.19.4009 |
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