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Functional Selection of shRNA Loops from Randomized Retroviral Libraries
Gene silencing by RNA interference (RNAi) can be achieved by the ectopic expression of tailored short hairpin RNAs (shRNAs) which after export to the cytoplasm are processed by Dicer and incorporated into the RNA induced silencing complex (RISC). Design rules for shRNAs have been the focus of severa...
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| Autores principales: | , , , , |
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| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
Public Library of Science
2012
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3422301/ https://ncbi.nlm.nih.gov/pubmed/22912797 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0043095 |
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