Functional Selection of shRNA Loops from Randomized Retroviral Libraries

Gene silencing by RNA interference (RNAi) can be achieved by the ectopic expression of tailored short hairpin RNAs (shRNAs) which after export to the cytoplasm are processed by Dicer and incorporated into the RNA induced silencing complex (RISC). Design rules for shRNAs have been the focus of severa...

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Bibliografiske detaljer
Main Authors: Jensen, Stig Mølgaard Rask, Schmitz, Alexander, Pedersen, Finn Skou, Kjems, Jørgen, Bramsen, Jesper Bertram
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science 2012
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3422301/
https://ncbi.nlm.nih.gov/pubmed/22912797
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0043095
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