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Introduction of Mismatches in a Random shRNA-Encoding Library Improves Potency for Phenotypic Selection
RNA interference (RNAi) is a mechanism for interfering with gene expression through the action of small, non-coding RNAs. We previously constructed a short-hairpin-loop RNA (shRNA) encoding library that is random at the nucleotide level [1]. In this library, the stems of the hairpin are completely c...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3911983/ https://ncbi.nlm.nih.gov/pubmed/24498319 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0087390 |
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