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Introduction of Mismatches in a Random shRNA-Encoding Library Improves Potency for Phenotypic Selection

RNA interference (RNAi) is a mechanism for interfering with gene expression through the action of small, non-coding RNAs. We previously constructed a short-hairpin-loop RNA (shRNA) encoding library that is random at the nucleotide level [1]. In this library, the stems of the hairpin are completely c...

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Hlavní autoři: Wang, Yongping, Speier, Jacqueline S., Engram-Pearl, Jessica, Wilson, Robert B.
Médium: Artigo
Jazyk:Inglês
Vydáno: Public Library of Science 2014
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3911983/
https://ncbi.nlm.nih.gov/pubmed/24498319
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0087390
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