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Introduction of Mismatches in a Random shRNA-Encoding Library Improves Potency for Phenotypic Selection

RNA interference (RNAi) is a mechanism for interfering with gene expression through the action of small, non-coding RNAs. We previously constructed a short-hairpin-loop RNA (shRNA) encoding library that is random at the nucleotide level [1]. In this library, the stems of the hairpin are completely c...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Wang, Yongping, Speier, Jacqueline S., Engram-Pearl, Jessica, Wilson, Robert B.
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: Public Library of Science 2014
Gaiak:
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC3911983/
https://ncbi.nlm.nih.gov/pubmed/24498319
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0087390
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