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Functional Selection of shRNA Loops from Randomized Retroviral Libraries
Gene silencing by RNA interference (RNAi) can be achieved by the ectopic expression of tailored short hairpin RNAs (shRNAs) which after export to the cytoplasm are processed by Dicer and incorporated into the RNA induced silencing complex (RISC). Design rules for shRNAs have been the focus of severa...
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| Hauptverfasser: | , , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Public Library of Science
2012
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3422301/ https://ncbi.nlm.nih.gov/pubmed/22912797 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0043095 |
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