Function-based gene identification using enzymatically generated normalized shRNA library and massive parallel sequencing

As a general strategy for function-based gene identification, an shRNA library containing ≈150 shRNAs per gene was enzymatically generated from normalized (reduced-redundance) human cDNA. The library was constructed in an inducible lentiviral vector, enabling propagation of growth-inhibiting shRNAs...

Descrizione completa

Salvato in:
Dettagli Bibliografici
Autori principali: Shtutman, Michael, Maliyekkel, Anil, Shao, Yu, Carmack, C. Steven, Baig, Mirza, Warholic, Natalie, Cole, Kelly, Broude, Eugenia V., Harkins, Timothy T., Ding, Ye, Roninson, Igor B.
Natura: Artigo
Lingua:Inglês
Pubblicazione: National Academy of Sciences 2010
Soggetti:
Biological Sciences
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2867740/
https://ncbi.nlm.nih.gov/pubmed/20368428
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1003055107
Tags: Aggiungi Tag
Nessun Tag, puoi essere il primo ad aggiungerne! !

Documenti analoghi