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HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

CCCTC-binding factor (CTCF)-mediated stable topologically associating domains (TADs) play a critical role in constraining interactions of DNA elements that are located in neighboring TADs. CTCF plays an important role in regulating the spatial and temporal expression of HOX genes that control embryo...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Luo, Huacheng, Sobh, Amin, Vulpe, Christopher D., Brewer, Edmond, Dovat, Sinisa, Qiu, Yi, Huang, Suming
Formato: Artigo
Idioma:Inglês
Publicado em: 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7607627/
https://ncbi.nlm.nih.gov/pubmed/30985763
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/59382
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