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Genome-scale CRISPR screening at high sensitivity with an empirically designed sgRNA library

BACKGROUND: In recent years, large-scale genetic screens using the CRISPR/Cas9 system have emerged as scalable approaches able to interrogate gene function with unprecedented efficiency and specificity in various biological contexts. By this means, functional dependencies on both the protein-coding...

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Detalhes bibliográficos
Publicado no:BMC Biol
Main Authors: Henkel, Luisa, Rauscher, Benedikt, Schmitt, Barbara, Winter, Jan, Boutros, Michael
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7686728/
https://ncbi.nlm.nih.gov/pubmed/33228647
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12915-020-00905-1
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