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Sequence determinants of improved CRISPR sgRNA design
The CRISPR/Cas9 system has revolutionized mammalian somatic cell genetics. Genome-wide functional screens using CRISPR/Cas9-mediated knockout or dCas9 fusion-mediated inhibition/activation (CRISPRi/a) are powerful techniques for discovering phenotype-associated gene function. We systematically asses...
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| Publicado no: | Genome Res |
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| Main Authors: | , , , , , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Cold Spring Harbor Laboratory Press
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4509999/ https://ncbi.nlm.nih.gov/pubmed/26063738 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.191452.115 |
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