Sequence determinants of improved CRISPR sgRNA design

The CRISPR/Cas9 system has revolutionized mammalian somatic cell genetics. Genome-wide functional screens using CRISPR/Cas9-mediated knockout or dCas9 fusion-mediated inhibition/activation (CRISPRi/a) are powerful techniques for discovering phenotype-associated gene function. We systematically asses...

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Podrobná bibliografie
Vydáno v:Genome Res
Hlavní autoři: Xu, Han, Xiao, Tengfei, Chen, Chen-Hao, Li, Wei, Meyer, Clifford A., Wu, Qiu, Wu, Di, Cong, Le, Zhang, Feng, Liu, Jun S., Brown, Myles, Liu, X. Shirley
Médium: Artigo
Jazyk:Inglês
Vydáno: Cold Spring Harbor Laboratory Press 2015
Témata:
Research
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4509999/
https://ncbi.nlm.nih.gov/pubmed/26063738
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.191452.115
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