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A systematic evaluation of nucleotide properties for CRISPR sgRNA design
BACKGROUND: CRISPR is a versatile gene editing tool which has revolutionized genetic research in the past few years. Optimizing sgRNA design to improve the efficiency of target/DNA cleavage is critical to ensure the success of CRISPR screens. RESULTS: By borrowing knowledge from oligonucleotide desi...
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| Udgivet i: | BMC Bioinformatics |
|---|---|
| Main Authors: | , , , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
BioMed Central
2017
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5461693/ https://ncbi.nlm.nih.gov/pubmed/28587596 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12859-017-1697-6 |
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