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CRISPR-Switch regulates sgRNA activity by Cre recombination for sequential editing of two loci
CRISPR-Cas9 is an efficient and versatile tool for genome engineering in many species. However, inducible CRISPR-Cas9 editing systems that regulate Cas9 activity or sgRNA expression often suffer from significant limitations, including reduced editing capacity, off-target effects, or leaky expression...
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| Publicado no: | Nat Commun |
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| Main Authors: | , , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Publishing Group UK
2019
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6884486/ https://ncbi.nlm.nih.gov/pubmed/31784531 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-019-13403-y |
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