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Design of a generic CRISPR-Cas9 approach using the same sgRNA to perform gene editing at distinct loci
Abstract Background The CRISPR/Cas (clustered regularly interspaced short palindromic repeat and CRISPR-associated nucleases) based technologies have revolutionized genome engineering. While their use for prokaryotic genome editing is expanding, some limitations remain such as possible off-target ef...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BMC
2019-03-01
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| Colecção: | BMC Biotechnology |
| Assuntos: | |
| Acesso em linha: | http://link.springer.com/article/10.1186/s12896-019-0509-7 |
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