Direct Delivery of Cas9-sgRNA Ribonucleoproteins into Cells Using a Nanoneedle Array

The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system is a powerful and widely used tool for genome editing. Recently, it was reported that direct delivery of Cas9-sgRNA ribonucleoproteins (RNPs) reduced off-target effects. Therefore, non-invasive, high-throughput method...

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Detalhes bibliográficos
Main Authors: Ayana Yamagishi, Daisuke Matsumoto, Yoshio Kato, Yuki Honda, Mone Morikawa, Futoshi Iwata, Takeshi Kobayashi, Chikashi Nakamura
Formato: Artigo
Idioma:Inglês
Publicado em: MDPI AG 2019-03-01
Colecção:Applied Sciences
Assuntos:
Genome editing
CRISPR/Cas9
Nanoneedle
Acesso em linha:http://www.mdpi.com/2076-3417/9/5/965
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