Optimized Cas9:sgRNA delivery efficiently generates biallelic MSTN knockout sheep without affecting meat quality

Abstract Background CRISPR/Cas9-based genome-editing systems have been used to efficiently engineer livestock species with precise genetic alterations intended for biomedical and agricultural applications. Previously, we have successfully generated gene-edited sheep and goats via one-cell-stage embr...

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Main Authors: Shiwei Zhou, Peter Kalds, Qi Luo, Kexin Sun, Xiaoe Zhao, Yawei Gao, Bei Cai, Shuhong Huang, Qifang Kou, Bjoern Petersen, Yulin Chen, Baohua Ma, Xiaolong Wang
Formato: Artigo
Idioma:Inglês
Publicado em: BMC 2022-05-01
Colecção:BMC Genomics
Assuntos:
Genome editing
CRISPR/Cas9 optimization
Homozygous gene knockout
Sheep
MSTN
Muscle growth
Acesso em linha:https://doi.org/10.1186/s12864-022-08594-6
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