CRISPR-Switch regulates sgRNA activity by Cre recombination for sequential editing of two loci

CRISPR-Cas9 is an efficient and versatile tool for genome engineering in many species. However, inducible CRISPR-Cas9 editing systems that regulate Cas9 activity or sgRNA expression often suffer from significant limitations, including reduced editing capacity, off-target effects, or leaky expression...

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Veröffentlicht in:Nat Commun
Hauptverfasser: Chylinski, Krzysztof, Hubmann, Maria, Hanna, Ruth E., Yanchus, Connor, Michlits, Georg, Uijttewaal, Esther C. H., Doench, John, Schramek, Daniel, Elling, Ulrich
Format: Artigo
Sprache:Inglês
Veröffentlicht: Nature Publishing Group UK 2019
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6884486/
https://ncbi.nlm.nih.gov/pubmed/31784531
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-019-13403-y
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