A Molecular Chipper technology for CRISPR sgRNA library generation and functional mapping of noncoding regions

Clustered regularly-interspaced palindromic repeats (CRISPR)-based genetic screens using single-guide-RNA (sgRNA) libraries have proven powerful to identify genetic regulators. Applying CRISPR screens to interrogate functional elements in noncoding regions requires generating sgRNA libraries that ar...

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Publicat a:Nat Commun
Autors principals: Cheng, Jijun, Roden, Christine A., Pan, Wen, Zhu, Shu, Baccei, Anna, Pan, Xinghua, Jiang, Tingting, Kluger, Yuval, Weissman, Sherman M., Guo, Shangqin, Flavell, Richard A., Ding, Ye, Lu, Jun
Format: Artigo
Idioma:Inglês
Publicat: Nature Publishing Group 2016
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Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4820989/
https://ncbi.nlm.nih.gov/pubmed/27025950
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms11178
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