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Quantile normalization of single-cell RNA-seq read counts without unique molecular identifiers

Single-cell RNA-seq (scRNA-seq) profiles gene expression of individual cells. Unique molecular identifiers (UMIs) remove duplicates in read counts resulting from polymerase chain reaction, a major source of noise. For scRNA-seq data lacking UMIs, we propose quasi-UMIs: quantile normalization of read...

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Bibliografske podrobnosti
izdano v:Genome Biol
Main Authors: Townes, F. William, Irizarry, Rafael A.
Format: Artigo
Jezik:Inglês
Izdano: BioMed Central 2020
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC7333325/
https://ncbi.nlm.nih.gov/pubmed/32620142
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13059-020-02078-0
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