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An efficient method for generation of Knock-out human embryonic stem cells using CRISPR/Cas9 system

Human embryonic stem cells (hESCs) represent promising tool to study functions of genes during development, to model diseases, and to even develop therapies when combined with gene editing techniques such as CRISPR/Cas9 system. However, the process of disruption of gene expression by generation of n...

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Bibliografiska uppgifter
I publikationen:Stem Cells Dev
Huvudupphovsmän: Bohaciakova, Dasa, Renzova, Tereza, Fedorova, Veronika, Barak, Martin, Kunova Bosakova, Michaela, Hampl, Ales, Cajanek, Lukas
Materialtyp: Artigo
Språk:Inglês
Publicerad: 2017
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC6328014/
https://ncbi.nlm.nih.gov/pubmed/28835165
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1089/scd.2017.0058
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