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An efficient method for generation of Knock-out human embryonic stem cells using CRISPR/Cas9 system

Human embryonic stem cells (hESCs) represent promising tool to study functions of genes during development, to model diseases, and to even develop therapies when combined with gene editing techniques such as CRISPR/Cas9 system. However, the process of disruption of gene expression by generation of n...

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Detalles Bibliográficos
Publicado en:Stem Cells Dev
Main Authors: Bohaciakova, Dasa, Renzova, Tereza, Fedorova, Veronika, Barak, Martin, Kunova Bosakova, Michaela, Hampl, Ales, Cajanek, Lukas
Formato: Artigo
Idioma:Inglês
Publicado: 2017
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC6328014/
https://ncbi.nlm.nih.gov/pubmed/28835165
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1089/scd.2017.0058
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