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An efficient method for generation of Knock-out human embryonic stem cells using CRISPR/Cas9 system

Human embryonic stem cells (hESCs) represent promising tool to study functions of genes during development, to model diseases, and to even develop therapies when combined with gene editing techniques such as CRISPR/Cas9 system. However, the process of disruption of gene expression by generation of n...

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Dettagli Bibliografici
Pubblicato in:Stem Cells Dev
Autori principali: Bohaciakova, Dasa, Renzova, Tereza, Fedorova, Veronika, Barak, Martin, Kunova Bosakova, Michaela, Hampl, Ales, Cajanek, Lukas
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2017
Soggetti:
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC6328014/
https://ncbi.nlm.nih.gov/pubmed/28835165
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1089/scd.2017.0058
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