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An efficient method for generation of Knock-out human embryonic stem cells using CRISPR/Cas9 system

Human embryonic stem cells (hESCs) represent promising tool to study functions of genes during development, to model diseases, and to even develop therapies when combined with gene editing techniques such as CRISPR/Cas9 system. However, the process of disruption of gene expression by generation of n...

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Bibliografski detalji
Izdano u:Stem Cells Dev
Glavni autori: Bohaciakova, Dasa, Renzova, Tereza, Fedorova, Veronika, Barak, Martin, Kunova Bosakova, Michaela, Hampl, Ales, Cajanek, Lukas
Format: Artigo
Jezik:Inglês
Izdano: 2017
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6328014/
https://ncbi.nlm.nih.gov/pubmed/28835165
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1089/scd.2017.0058
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