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CRISPR/Cas9 + AAV-mediated Intra-embryonic Gene Knocking in Mice
Intra-embryo genome editing by CRISPR/Cas9 has enabled rapid generation of gene knockout animals. However, large fragment knock-in directly into embryos’ genome is still difficult, especially without microinjection of donor DNA. Viral vectors are good transporters of knock-in donor DNA for cell line...
Uloženo v:
| Vydáno v: | Bio Protoc |
|---|---|
| Hlavní autoři: | , , , , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
Bio-Protocol
2019
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7854279/ https://ncbi.nlm.nih.gov/pubmed/33654808 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3295 |
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