CRISPR/Cas9-mediated reporter knock-in in mouse haploid embryonic stem cells

Mouse parthenogenetic haploid embryonic stem cells (ESCs) are pluripotent cells generated from chemically activated oocytes. Haploid ESCs provide an opportunity to study the effect of genetic alterations because of their hemizygotic characteristics. However, their further application for the selecti...

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Publicado en:Sci Rep
Autores principales: Kimura, Yasuyoshi, Oda, Masaaki, Nakatani, Tsunetoshi, Sekita, Yoichi, Monfort, Asun, Wutz, Anton, Mochizuki, Hideki, Nakano, Toru
Formato: Artigo
Lenguaje:Inglês
Publicado: Nature Publishing Group 2015
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Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4454075/
https://ncbi.nlm.nih.gov/pubmed/26039937
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep10710
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