CRISPR/Cas9-mediated reporter knock-in in mouse haploid embryonic stem cells

Mouse parthenogenetic haploid embryonic stem cells (ESCs) are pluripotent cells generated from chemically activated oocytes. Haploid ESCs provide an opportunity to study the effect of genetic alterations because of their hemizygotic characteristics. However, their further application for the selecti...

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Udgivet i:Sci Rep
Main Authors: Kimura, Yasuyoshi, Oda, Masaaki, Nakatani, Tsunetoshi, Sekita, Yoichi, Monfort, Asun, Wutz, Anton, Mochizuki, Hideki, Nakano, Toru
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group 2015
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4454075/
https://ncbi.nlm.nih.gov/pubmed/26039937
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep10710
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