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CRISPR/Cas9-mediated reporter knock-in in mouse haploid embryonic stem cells

Mouse parthenogenetic haploid embryonic stem cells (ESCs) are pluripotent cells generated from chemically activated oocytes. Haploid ESCs provide an opportunity to study the effect of genetic alterations because of their hemizygotic characteristics. However, their further application for the selecti...

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Détails bibliographiques
Publié dans:Sci Rep
Auteurs principaux: Kimura, Yasuyoshi, Oda, Masaaki, Nakatani, Tsunetoshi, Sekita, Yoichi, Monfort, Asun, Wutz, Anton, Mochizuki, Hideki, Nakano, Toru
Format: Artigo
Langue:Inglês
Publié: Nature Publishing Group 2015
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4454075/
https://ncbi.nlm.nih.gov/pubmed/26039937
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep10710
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