CRISPR/Cas9-mediated reporter knock-in in mouse haploid embryonic stem cells

Mouse parthenogenetic haploid embryonic stem cells (ESCs) are pluripotent cells generated from chemically activated oocytes. Haploid ESCs provide an opportunity to study the effect of genetic alterations because of their hemizygotic characteristics. However, their further application for the selecti...

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Bibliografski detalji
Izdano u:Sci Rep
Glavni autori: Kimura, Yasuyoshi, Oda, Masaaki, Nakatani, Tsunetoshi, Sekita, Yoichi, Monfort, Asun, Wutz, Anton, Mochizuki, Hideki, Nakano, Toru
Format: Artigo
Jezik:Inglês
Izdano: Nature Publishing Group 2015
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Article
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4454075/
https://ncbi.nlm.nih.gov/pubmed/26039937
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep10710
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