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Optimized knock-in of point mutations in zebrafish using CRISPR/Cas9
We have optimized point mutation knock-ins into zebrafish genomic sites using clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 reagents and single-stranded oligodeoxynucleotides. The efficiency of knock-ins was assessed by a novel application of allele-specific polymerase chain reac...
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| Pubblicato in: | Nucleic Acids Res |
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| Autori principali: | , , , , , , , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Oxford University Press
2018
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6158492/ https://ncbi.nlm.nih.gov/pubmed/29905858 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gky512 |
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