Optimized knock-in of point mutations in zebrafish using CRISPR/Cas9

We have optimized point mutation knock-ins into zebrafish genomic sites using clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 reagents and single-stranded oligodeoxynucleotides. The efficiency of knock-ins was assessed by a novel application of allele-specific polymerase chain reac...

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Veröffentlicht in:Nucleic Acids Res
Hauptverfasser: Prykhozhij, Sergey V, Fuller, Charlotte, Steele, Shelby L, Veinotte, Chansey J, Razaghi, Babak, Robitaille, Johane M, McMaster, Christopher R, Shlien, Adam, Malkin, David, Berman, Jason N
Format: Artigo
Sprache:Inglês
Veröffentlicht: Oxford University Press 2018
Schlagworte:
Methods Online
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6158492/
https://ncbi.nlm.nih.gov/pubmed/29905858
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gky512
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