Generation of beta-lactoglobulin knock-out goats using CRISPR/Cas9

Goat’s milk, considered a substitute for cow’s milk, has a high nutritional value. However, goat’s milk contains various allergens, predominantly β-lactoglobulin (BLG). In this study, we employed the CRISPR/Cas9 system to target the BLG locus in goat fibroblasts for sgRNA optimization and generate B...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Zhou, Wenjun, Wan, Yongjie, Guo, Rihong, Deng, Mingtian, Deng, Kaiping, Wang, Zhen, Zhang, Yanli, Wang, Feng
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2017
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5634636/
https://ncbi.nlm.nih.gov/pubmed/29016691
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0186056
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