Activities and specificities of CRISPR/Cas9 and Cas12a nucleases for targeted mutagenesis in maize

CRISPR/Cas9 and Cas12a (Cpf1) nucleases are two of the most powerful genome editing tools in plants. In this work, we compared their activities by targeting maize glossy2 gene coding region that has overlapping sequences recognized by both nucleases. We introduced constructs carrying SpCas9‐guide RN...

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Dettagli Bibliografici
Pubblicato in:Plant Biotechnol J
Autori principali: Lee, Keunsub, Zhang, Yingxiao, Kleinstiver, Benjamin P., Guo, Jimmy A., Aryee, Martin J., Miller, Jonah, Malzahn, Aimee, Zarecor, Scott, Lawrence‐Dill, Carolyn J., Joung, J. Keith, Qi, Yiping, Wang, Kan
Natura: Artigo
Lingua:Inglês
Pubblicazione: John Wiley and Sons Inc. 2018
Soggetti:
Research Articles
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC6320322/
https://ncbi.nlm.nih.gov/pubmed/29972722
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/pbi.12982
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