Activities and specificities of CRISPR/Cas9 and Cas12a nucleases for targeted mutagenesis in maize

CRISPR/Cas9 and Cas12a (Cpf1) nucleases are two of the most powerful genome editing tools in plants. In this work, we compared their activities by targeting maize glossy2 gene coding region that has overlapping sequences recognized by both nucleases. We introduced constructs carrying SpCas9‐guide RN...

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Bibliographic Details
Published in:Plant Biotechnol J
Main Authors: Lee, Keunsub, Zhang, Yingxiao, Kleinstiver, Benjamin P., Guo, Jimmy A., Aryee, Martin J., Miller, Jonah, Malzahn, Aimee, Zarecor, Scott, Lawrence‐Dill, Carolyn J., Joung, J. Keith, Qi, Yiping, Wang, Kan
Format: Artigo
Language:Inglês
Published: John Wiley and Sons Inc. 2018
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Research Articles
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6320322/
https://ncbi.nlm.nih.gov/pubmed/29972722
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/pbi.12982
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