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Defining CRISPR-Cas9 genome-wide nuclease activities with CIRCLE-seq

Circularization for in vitro reporting of cleavage effects by sequencing (CIRCLE-seq) is a sensitive and unbiased method for defining the genome-wide activity (on-target and off-target) of CRISPR-Cas9 nucleases by selective sequencing of nuclease-cleaved genomic DNA. Here we describe a detailed expe...

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書目詳細資料
發表在:Nat Protoc
Main Authors: Lazzarotto, Cicera R., Nguyen, Nhu T., Tang, Xing, Malagon-Lopez, Jose, Guo, Jimmy, Aryee, Martin J., Joung, J. Keith, Tsai, Shengdar Q.
格式: Artigo
語言:Inglês
出版: 2018
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC6512799/
https://ncbi.nlm.nih.gov/pubmed/30341435
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41596-018-0055-0
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