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Defining CRISPR-Cas9 genome-wide nuclease activities with CIRCLE-seq
Circularization for in vitro reporting of cleavage effects by sequencing (CIRCLE-seq) is a sensitive and unbiased method for defining the genome-wide activity (on-target and off-target) of CRISPR-Cas9 nucleases by selective sequencing of nuclease-cleaved genomic DNA. Here we describe a detailed expe...
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| Publicado no: | Nat Protoc |
|---|---|
| Main Authors: | , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6512799/ https://ncbi.nlm.nih.gov/pubmed/30341435 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41596-018-0055-0 |
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