CIRCLE-seq: a highly sensitive in vitro screen for genome-wide CRISPR-Cas9 nuclease off-targets

Sensitive detection of off-target effects is important for translating CRISPR-Cas9 nucleases into human therapeutics. In vitro biochemical methods for finding off-targets offer potential advantages of greater reproducibility and scalability while avoiding limitations associated with strategies that...

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Bibliographic Details
Published in:Nat Methods
Main Authors: Tsai, Shengdar Q., Nguyen, Nhu T., Malagon-Lopez, Jose, Topkar, Ved V., Aryee, Martin J., Joung, J. Keith
Format: Artigo
Language:Inglês
Published: 2017
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5924695/
https://ncbi.nlm.nih.gov/pubmed/28459458
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.4278
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