GUIDE-Seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases

CRISPR RNA-guided nucleases (RGNs) are widely used genome-editing reagents, but methods to delineate their genome-wide off-target cleavage activities have been lacking. Here we describe an approach for global detection of DNA double-stranded breaks (DSBs) introduced by RGNs and potentially other nuc...

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Gepubliceerd in:Nat Biotechnol
Hoofdauteurs: Tsai, Shengdar Q., Zheng, Zongli, Nguyen, Nhu T., Liebers, Matthew, Topkar, Ved V., Thapar, Vishal, Wyvekens, Nicolas, Khayter, Cyd, Iafrate, A. John, Le, Long P., Aryee, Martin J., Joung, J. Keith
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 2014
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Article
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4320685/
https://ncbi.nlm.nih.gov/pubmed/25513782
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nbt.3117
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