GUIDE-Seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases

CRISPR RNA-guided nucleases (RGNs) are widely used genome-editing reagents, but methods to delineate their genome-wide off-target cleavage activities have been lacking. Here we describe an approach for global detection of DNA double-stranded breaks (DSBs) introduced by RGNs and potentially other nuc...

ver descrição completa

Na minha lista:
Detalhes bibliográficos
Publicado no:Nat Biotechnol
Main Authors: Tsai, Shengdar Q., Zheng, Zongli, Nguyen, Nhu T., Liebers, Matthew, Topkar, Ved V., Thapar, Vishal, Wyvekens, Nicolas, Khayter, Cyd, Iafrate, A. John, Le, Long P., Aryee, Martin J., Joung, J. Keith
Formato: Artigo
Idioma:Inglês
Publicado em: 2014
Assuntos:
Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4320685/
https://ncbi.nlm.nih.gov/pubmed/25513782
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nbt.3117
Tags: Adicionar Tag
Sem tags, seja o primeiro a adicionar uma tag!

Registos relacionados