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Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human cells

The activities and genome-wide specificities of CRISPR-Cas Cpf1 nucleases(1) are not well defined. We show that two Cpf1 nucleases from Acidaminococcus sp. BV3L6 and Lachnospiraceae bacterium ND2006 (AsCpf1 and LbCpf1, respectively) have on-target efficiencies in human cells comparable with those of...

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Bibliografski detalji
Izdano u:Nat Biotechnol
Glavni autori: Kleinstiver, Benjamin P., Tsai, Shengdar Q., Prew, Michelle S., Nguyen, Nhu T., Welch, Moira M., Lopez, Jose M., McCaw, Zachary R., Aryee, Martin J., Joung, J. Keith
Format: Artigo
Jezik:Inglês
Izdano: 2016
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4980201/
https://ncbi.nlm.nih.gov/pubmed/27347757
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nbt.3620
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