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High-fidelity CRISPR-Cas9 variants with undetectable genome-wide off-targets
CRISPR-Cas9 nucleases are widely used for genome editing but can induce unwanted off-target mutations. Existing strategies for reducing genome-wide off-targets of the broadly used Streptococcus pyogenes Cas9 (SpCas9) are imperfect, possessing only partial or unproven efficacies and other limitations...
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| Udgivet i: | Nature |
|---|---|
| Main Authors: | , , , , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2016
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4851738/ https://ncbi.nlm.nih.gov/pubmed/26735016 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nature16526 |
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