Broadening Staphylococcus aureus Cas9 Targeting Range by Modifying PAM Recognition

CRISPR-Cas9 nucleases are primarily guided by RNA-DNA interactions but also require Cas9-mediated recognition of a protospacer adjacent motif (PAM). While potentially advantageous for specificity, extended PAM sequences limit the targeting range of Cas9 orthologues for genome editing. One possible s...

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Bibliografiska uppgifter
I publikationen:Nat Biotechnol
Huvudupphovsmän: Kleinstiver, Benjamin P., Prew, Michelle S., Tsai, Shengdar Q., Nguyen, Nhu T., Topkar, Ved V., Zheng, Zongli, Joung, J. Keith
Materialtyp: Artigo
Språk:Inglês
Publicerad: 2015
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Article
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC4689141/
https://ncbi.nlm.nih.gov/pubmed/26524662
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nbt.3404
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