Application of CRISPR-Cas12a temperature sensitivity for improved genome editing in rice, maize, and Arabidopsis

Abstract Background CRISPR-Cas12a (formerly Cpf1) is an RNA-guided endonuclease with distinct features that have expanded genome editing capabilities. Cas12a-mediated genome editing is temperature sensitive in plants, but a lack of a comprehensive understanding on Cas12a temperature sensitivity in p...

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Hauptverfasser: Aimee A. Malzahn, Xu Tang, Keunsub Lee, Qiurong Ren, Simon Sretenovic, Yingxiao Zhang, Hongqiao Chen, Minjeong Kang, Yu Bao, Xuelian Zheng, Kejun Deng, Tao Zhang, Valeria Salcedo, Kan Wang, Yong Zhang, Yiping Qi
Format: Artigo
Sprache:Inglês
Veröffentlicht: BMC 2019-01-01
Schriftenreihe:BMC Biology
Schlagworte:
CRISPR-Cas12a
Temperature
Genome editing
Rice
Arabidopsis
Maize
Online Zugang:http://link.springer.com/article/10.1186/s12915-019-0629-5
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